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Addgene

pBabe bleo IRES-EGFP
(Plasmid #14432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe bleo
  • Backbone size w/o insert (bp) 4983
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IRES-EGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    1331

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaB1, EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBabe vectors used to construct these EGFP expressing versions were described by Morgenstern and Land in NAR, Vol 18, No 12, pp3587 (1990), if you generate a publication using these vectors, please acknowledge L. Miguel Martins

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe bleo IRES-EGFP was a gift from L. Miguel Martins (Addgene plasmid # 14432 ; http://n2t.net/addgene:14432 ; RRID:Addgene_14432)
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