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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe neo
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIRES-EGFP
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SpeciesAequorea victoria
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Insert Size (bp)1331
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaB1, EcoRI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBabe vectors used to construct these EGFP expressing versions were described by Morgenstern and Land in NAR, Vol 18, No 12, pp3587 (1990), if you generate a publication using these vectors, please acknowledge L. Miguel Martins
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe neo IRES-EGFP was a gift from L. Miguel Martins (Addgene plasmid # 14431 ; http://n2t.net/addgene:14431 ; RRID:Addgene_14431)