pBabe puro IRES-EGFP
(Plasmid #14430)

• Depositing Lab: L. Miguel Martins
• Publication: pBabe-IRES-EGFP plasmids (unpublished)

Backbone

• Vector backbone: pBabe puro
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IRES-EGFP
• Species: Aequorea victoria
• Insert Size (bp): 1331

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • SnaBI site does not exist
  • Addgene Diagnostic Digest for 14430
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBabe vectors used to construct these EGFP expressing versions were described by Morgenstern and Land in NAR, Vol 18, No 12, pp3587 (1990), if you generate a publication using these vectors, please acknowledge L. Miguel Martins

Please note that Addgene's sequencing results show an XhoI site in place of the SnaBI site. See Notes link for more information.

Information for Cloning Grade DNA (Catalog # 14430-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here