pAd-Track Flag HA PGC-1 alpha
(Plasmid #14426)

• Depositing Lab: Pere Puigserver
• Publication: Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Backbone

• Vector backbone: pAdTrack-CMV
• Backbone size w/o insert (bp): 9000
• Vector type: Mammalian Expression, Adenoviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGC-1 alpha
• Alt name: PPAR gamma coactivator 1
• Alt name: PGC1
• Species: M. musculus (mouse)
• Insert Size (bp): 2400
• Mutation: Last four amino acids of PGC-1a removed to make the protein more stable.
• GenBank ID: NM_008904
• Entrez Gene: Ppargc1a (a.k.a. A830037N07Rik, Gm11133, PGC-1, PPARGC-1-alpha, Pgc-1alpha, Pgc1, Pgco1, Ppargc1)
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pAdTrack can be recombined with pAdEasy to make adenoviral-PGC1.

See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.