pAd-Track Flag GCN5 Y621A/P622A
(Plasmid #14425)

• Depositing Lab: Pere Puigserver
• Publication: Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Backbone

• Vector backbone: pAdTrack
• Backbone size w/o insert (bp): 9000
• Vector type: Mammalian Expression, Adenoviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCN5 Y621A/P622A
• Alt name: GCN5
• Species: H. sapiens (human)
• Mutation: Y621A, P622A. Catalytically inactive acetyltransferase mutant
• Entrez Gene: KAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The catalytically inactive acetyltransferase mutant GCN5 (Y621A/P622A) (GCN5m) was generated by site-directed mutagenesis and verified by DNA sequencing.

pAdTrack can be recombined with pAdEasy to make adenoviral-GCN5m.
See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.

Please note that Addgene quality control sequencing confirmed 2 additional mutations (E189G and K195N) which do not alter the plasmid's function.