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Addgene

pAd-Track Flag GCN5
(Plasmid #14424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14424 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAdTrack
  • Backbone size w/o insert (bp) 9000
  • Vector type
    Mammalian Expression, Adenoviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCN5
  • Species
    H. sapiens (human)
  • Entrez Gene
    KAT2A (a.k.a. GCN5, GCN5L2, PCAF-b, hGCN5)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Flag-GCN5 was cloned into the pAdTrack shuttle vector. Insert = KpnI - FLAG - EcoRI - GCN5 - XhoI.

pAdTrack can be recombined with pAdEasy to make adenoviral-GCN5.

See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd-Track Flag GCN5 was a gift from Pere Puigserver (Addgene plasmid # 14424)

  • For your References section:

    GCN5 acetyltransferase complex controls glucose metabolism through transcriptional repression of PGC-1alpha. Lerin C, Rodgers JT, Kalume DE, Kim SH, Pandey A, Puigserver P. Cell Metab. 2006 Jun . 3(6):429-38. 10.1016/j.cmet.2006.04.013 PubMed 16753578
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