pU6-STOP-shA1
(Plasmid #14422)

• Depositing Lab: Klaus Rajewsky
• Publication: Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-905.

Backbone

• Vector backbone: pBS-cas2
• Backbone manufacturer: Rene Maehr
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
• Species: M. musculus (mouse)
• Insert Size (bp): 633

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (unknown if destroyed)
• 3′ cloning site: AscI (unknown if destroyed)
• 5′ sequencing primer: M13 forward or M13 reverse
• 3′ sequencing primer: M13 forward or M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

inducible shRNA expression vector. shRNA coding region can be replaced as follows: design ~80 bp oligos with 5’ BamHI site followed by mutant loxP, where the last 5 bp are replaced by the first 5 bp of shRNA sequence, followed by the remaining shRNA sequence, hairpin and as shRNA sequence, T stretch and SalI site.