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Addgene

TFORF2185
(Plasmid #143574)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 143574 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX_TRC317
  • Backbone manufacturer
    Broad Institute Genetic Perturbation Platform
  • Backbone size w/o insert (bp) 8264
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF414
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1040
  • Entrez Gene
    ZNF414 (a.k.a. ZFP414, MGC15716)
  • Promoter EF1a

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GGGTGGAGACTGAAGTTAGGCCAG
  • 3′ sequencing primer CACATAGCGTAAAAGGAGCAACATAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NM_032370, ENST00000255616. The transcription factor ORF portion of this plasmid was synthesized by Genewiz.

Addgene Notes: Please test multiple small colonies in case of plasmid recombination. A portion of the backbone is duplicated (1421 - 2371 bp and 2366 - 3316 bp), however the plasmid still functions as expected.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TFORF2185 was a gift from Feng Zhang (Addgene plasmid # 143574 ; http://n2t.net/addgene:143574 ; RRID:Addgene_143574)

  • For your References section:

    A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell. 2023 Jan 5;186(1):209-229.e26. doi: 10.1016/j.cell.2022.11.026. 10.1016/j.cell.2022.11.026 PubMed 36608654
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