pAG423GPD-ccdB-HA
(Plasmid #14246)

• Purpose: (Empty Backbone)
• Depositing Lab: Susan Lindquist
• Publication: Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Backbone

• Vector backbone: pRS423
• Vector type: Yeast Expression ; Gateway Destination
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: DB3.1, ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • 3HA (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GPDPro_F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Type of plasmid: 2-micron.

This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com

Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.