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Addgene

pAG415GAL-ccdB-HA Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: EcoRI and EcoRI/XbaI

We expected the following band sizes for this digest: 4.8kb, 2.4kb and 1.3kb for EcoRI and 4.4kb, 2.4kb, 1.3kb and 0.5kb for EcoRI/XbaI. We see the expected pattern.