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Addgene

pAG303GPD-ccdB-EGFP
(Plasmid #14182)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14182 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS303
  • Vector type
    Yeast Expression ; Gateway Destination
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1, ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Type of plasmid: integrating.

This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com

Please note that this plasmid is missing a CYC1 terminator. It is unknown how this will affect gene expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG303GPD-ccdB-EGFP was a gift from Susan Lindquist (Addgene plasmid # 14182 ; http://n2t.net/addgene:14182 ; RRID:Addgene_14182)

  • For your References section:

    A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae. Alberti S, Gitler AD, Lindquist S. Yeast. 2007 Oct . 24(10):913-9. 10.1002/yea.1502 PubMed 17583893
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