pBabeHygro-GATA2-Ala(381-385)
(Plasmid
#1416)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1416 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBabeHygro
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGATA-2
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Alt nameGATA2
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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Mutationalanine substitutions at amino acids 381-385
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GenBank IDNM_032638
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Entrez GeneGATA2 (a.k.a. DCML, IMD21, MONOMAC, NFE1B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site HpaI (destroyed during cloning)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabeHygro-GATA2-Ala(381-385) was a gift from Gokhan Hotamisligil (Addgene plasmid # 1416 ; http://n2t.net/addgene:1416 ; RRID:Addgene_1416) -
For your References section:
Interaction between GATA and the C/EBP family of transcription factors is critical in GATA-mediated suppression of adipocyte differentiation. Tong Q, Tsai J, Tan G, Dalgin G, Hotamisligil GS. Mol Cell Biol 2005 Jan;25(2):706-15. 10.1128/MCB.25.2.706-715.2005 PubMed 15632071