pAG426GAL-ccdB
(Plasmid #14155)

• Purpose: (Empty Backbone)
• Depositing Lab: Susan Lindquist
• Publication: Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Backbone

• Vector backbone: pRS426
• Vector type: Yeast Expression ; Gateway Destination
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: DB3.1, ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GAL1

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Type of plasmid: 2-micron.

This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com

Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.

Information for Cloning Grade DNA (Catalog # 14155-DNA.cg) ( Back to top)

Item is discontinued.

Addgene no longer distributes this item. Contact for more information.

2 µg of cloning grade DNA in Tris buffer

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here