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Addgene

pBabeHygro-GATA2-D415-475
(Plasmid #1415)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1415 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabeHygro
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GATA-2
  • Alt name
    GATA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Mutation
    deleted amino acids 415 to 475
  • GenBank ID
    NM_032638
  • Entrez Gene
    GATA2 (a.k.a. DCML, IMD21, MONOMAC, NFE1B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (not destroyed)
  • 3′ cloning site SnaBI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Current NCBI records (NP_116027.2) deleted aa420-480.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabeHygro-GATA2-D415-475 was a gift from Gokhan Hotamisligil (Addgene plasmid # 1415 ; http://n2t.net/addgene:1415 ; RRID:Addgene_1415)

  • For your References section:

    Interaction between GATA and the C/EBP family of transcription factors is critical in GATA-mediated suppression of adipocyte differentiation. Tong Q, Tsai J, Tan G, Dalgin G, Hotamisligil GS. Mol Cell Biol 2005 Jan;25(2):706-15. 10.1128/MCB.25.2.706-715.2005 PubMed 15632071
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