Skip to main content
Addgene

pMBP-HRV3C-AcrIF9
(Plasmid #141442)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMAL-c4x
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6645
  • Total vector size (bp) 6850
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AcrIF9
  • Alt name
    AcrF9
  • Alt name
    F9
  • Species
    Proteus penneri ATCC 35198
  • Insert Size (bp)
    207
  • GenBank ID
    EEG86164.1
  • Promoter ptac
  • Tags / Fusion Proteins
    • Maltose Binding Protein (N terminal on backbone)
    • HRV3C cleavage site (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMBP-HRV3C-AcrIF9 was a gift from Blake Wiedenheft (Addgene plasmid # 141442 ; http://n2t.net/addgene:141442 ; RRID:Addgene_141442)

  • For your References section:

    AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex. Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B. Nat Commun. 2020 Jun 1;11(1):2730. doi: 10.1038/s41467-020-16512-1. 10.1038/s41467-020-16512-1 PubMed 32483187
Related items: