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Yeo Lab RNA-Binding Protein Pooled CRISPR Knockout Library
(Pooled Library #141438)

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  • Purpose

    This pooled CRISPR knockout library targets RNA-binding proteins with 10 guide RNAs per target.

  • Vector Backbone

    lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 141438 RBP CRISPR Knockout Library 1 $540 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    1,078
  • gRNAs
    12,472
  • Controls


    Non-targeting controls: 1,058
    Essential genes: 628
    Fluorescent controls: 12

  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RNA-Binding Protein Pooled CRISPR Knockout Library was a gift from Eugene Yeo (Addgene #141438)

  • For your References section:

    Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Wheeler EC, Vu AQ, Einstein JM, DiSalvo M, Ahmed N, Van Nostrand EL, Shishkin AA, Jin W, Allbritton NL, Yeo GW. Nat Methods. 2020 May 11. pii: 10.1038/s41592-020-0826-8. doi: 10.1038/s41592-020-0826-8. PubMed 32393832
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