Superti-Furga Lab Mouse SLC Knockout Library
(Pooled Library #141436)

• Purpose:

  This CRISPR knockout library targets the mouse solute carrier family of proteins.

• Vector Backbone:

  lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

• Depositing Labs: Giulio Superti-Furga
• Publication: Girardi et al unpublished.

Library Details

• Species: Mouse
• Genes targeted: 378
• gRNAs: 2,384
• Controls: 119 non-targeting sgRNAs
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (PDF, 30 KB)
  • Library Sequencing Protocol (PDF, 93 KB)
• Depositor Data:
  • CRISPR Library NGS Primers (XLSX, 18 KB)
  • List of gRNA Sequences (FASTA, 103 KB)
  • Table of NGS Read Counts (TSV, 61 KB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

The library results can be analyzed with several packages, including the MAGeCK algorithms or R/DESeq.

The Research Empowerment on Solute Carriers (ReSOLUTE) plasmid collection contains codon-optimized human SLCs in Gateway-compatible pENTR vectors and may be a useful resource for researchers using this library.