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Addgene

FUW-tetO-NFIA
(Plasmid #141403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUW-tetO
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFIA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1530
  • Entrez Gene
    NFIA (a.k.a. BRMUTD, CTF, NF-I/A, NF1-A, NFI-A, NFI-L)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer FUW_F aactatgcgctcggg
  • 3′ sequencing primer FUW_R taaagcagcgtatcc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FUW-tetO-GFP (Addgene 30130) was digested with EcoRI to remove the GFP fragment and NFIA was inserted

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW-tetO-NFIA was a gift from Lorenz Studer (Addgene plasmid # 141403 ; http://n2t.net/addgene:141403 ; RRID:Addgene_141403)

  • For your References section:

    NFIA is a gliogenic switch enabling rapid derivation of functional human astrocytes from pluripotent stem cells. Tchieu J, Calder EL, Guttikonda SR, Gutzwiller EM, Aromolaran KA, Steinbeck JA, Goldstein PA, Studer L. Nat Biotechnol. 2019 Mar;37(3):267-275. doi: 10.1038/s41587-019-0035-0. Epub 2019 Feb 25. 10.1038/s41587-019-0035-0 PubMed 30804533
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