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Addgene

pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro with the following enzymes: EcoRI-HF and MluI-HF.

Expected Bands
EcoRI-HF: 9738 bp
MluI-HF: 9738 bp
EcoRI-HF/ MluI-HF: 7579 bp & 2159 bp

We observe the expected bands for this digest.