pBOB-CAG-SARS-CoV2-Spike-HA
(Plasmid #141347)

• Purpose: 3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
• Depositing Lab: Gerald Pao
• Publication: Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.07.21.214932.

Backbone

• Vector backbone: pBOB-CAG
• Backbone manufacturer: Verma Lab (Salk Institute)
• Total vector size (bp): 13475
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: do not overgrow
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV2 Spike Glycoprotein
• Alt name: Spike
• Alt name: S
• Species: SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID)
• Insert Size (bp): 3858
• Mutation: resynthesized with human codon optimization nucleotide sequence does not match original sequence.
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CAG
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CAG-FWD AGCCTCTGCTAACCATGTTC
• 3′ sequencing primer: WPRE-REV TCCTCCTCCTCTTGTGCTTC

Resource Information

• Supplemental Documents:
  • Western blot from transient and viral expression in cells
  • Immunofluorescence in infected cells
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry