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Addgene

GB1-PRD(703-800)-Strep
(Plasmid #141344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone size w/o insert (bp) 5643
  • Total vector size (bp) 6238
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GB1-His6-TEV-ALIX(703-800)-Strep
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    595
  • Tags / Fusion Proteins
    • GB1-His6-TEV (N terminal on insert)
    • Strep-tag (WSHPQFEK) (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB1-PRD(703-800)-Strep was a gift from Lalit Deshmukh (Addgene plasmid # 141344 ; http://n2t.net/addgene:141344 ; RRID:Addgene_141344)

  • For your References section:

    Proline-rich domain of human ALIX contains multiple TSG101-UEV interaction sites and forms phosphorylation-mediated reversible amyloids. Elias RD, Ma W, Ghirlando R, Schwieters CD, Reddy VS, Deshmukh L. Proc Natl Acad Sci U S A. 2020 Sep 11. pii: 2010635117. doi: 10.1073/pnas.2010635117. 10.1073/pnas.2010635117 PubMed 32917811
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