pLD-puro-Cn-VA-NHEJ1-S262A/S263A
(Plasmid
#141343)
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PurposeLentiviral vector for expression of human NHEJ1-S262A/S263A in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLD-puro-CnVA
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Backbone manufacturerAddgene # 24587
- Backbone size w/o insert (bp) 9418
- Total vector size (bp) 8598
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsGrow for no longer than 16 hr
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNHEJ1-S262A/S263A
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SpeciesH. sapiens (human)
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Insert Size (bp)1233
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MutationS262A/S263A
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GenBank ID79840
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Entrez GeneNHEJ1 (a.k.a. XLF)
- Promoter CMV
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Tag
/ Fusion Protein
- VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon) (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BshTI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer 5'-TCTTTCCCCTGCACTGTACC-3' (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMGC: 32656, IMAGE: 4076851, GenBank: BC030986.1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLD-puro-Cn-VA-NHEJ1-S262A/S263A was a gift from Mohan Babu (Addgene plasmid # 141343 ; http://n2t.net/addgene:141343 ; RRID:Addgene_141343) -
For your References section:
The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF. Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA, Golshani A, Babu M. Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585. 10.1093/nar/gkab585 PubMed 34244791