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pLD-puro-Cn-VA-NHEJ1-S262E/S263E
(Plasmid #141342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLD-puro-CnVA
  • Backbone manufacturer
    Addgene # 24587
  • Backbone size w/o insert (bp) 9418
  • Total vector size (bp) 8598
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow for no longer than 16 hr
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NHEJ1-S262E/S263E
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1233
  • Mutation
    S262E/S263E
  • GenBank ID
    79840
  • Entrez Gene
    NHEJ1 (a.k.a. XLF)
  • Promoter CMV
  • Tag / Fusion Protein
    • VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BshTI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer 5'-TCTTTCCCCTGCACTGTACC-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLD-puro-Cn-VA-NHEJ1-S262E/S263E was a gift from Mohan Babu (Addgene plasmid # 141342 ; http://n2t.net/addgene:141342 ; RRID:Addgene_141342)

  • For your References section:

    The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF. Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA, Golshani A, Babu M. Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585. 10.1093/nar/gkab585 PubMed 34244791
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