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Addgene

pLD-puro-Cc-TARDBP-A315T_VA
(Plasmid #141329)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141329 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLD-puro-Cc-VA (MAPLE vector)
  • Backbone manufacturer
    Addgene# 24588
  • Backbone size w/o insert (bp) 9389
  • Total vector size (bp) 8983
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow for no longer than 16 hr
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TARDBP-A315T
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1608
  • Mutation
    A315T
  • GenBank ID
    23435
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Tag / Fusion Protein
    • VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG) (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer 5'-TCTTTCCCCTGCACTGTACC-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLD-puro-Cc-TARDBP-A315T_VA was a gift from Mohan Babu (Addgene plasmid # 141329 ; http://n2t.net/addgene:141329 ; RRID:Addgene_141329)

  • For your References section:

    BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain. Pourhaghighi R, Ash PEA, Phanse S, Goebels F, Hu LZM, Chen S, Zhang Y, Wierbowski SD, Boudeau S, Moutaoufik MT, Malty RH, Malolepsza E, Tsafou K, Nathan A, Cromar G, Guo H, Abdullatif AA, Apicco DJ, Becker LA, Gitler AD, Pulst SM, Youssef A, Hekman R, Havugimana PC, White CA, Blum BC, Ratti A, Bryant CD, Parkinson J, Lage K, Babu M, Yu H, Bader GD, Wolozin B, Emili A. Cell Syst. 2020 Apr 22;10(4):333-350.e14. doi: 10.1016/j.cels.2020.03.003. 10.1016/j.cels.2020.03.003 PubMed 32325033
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