pET-28b-RfxCas13d-His
(Plasmid #141322)

• Purpose: Plasmid for bacterial expression and purification of RfxCas13d protein
• Depositing Labs: Ariel Bazzini, Miguel Angel Moreno-Mateos
• Publication: Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7. doi: 10.1016/j.devcel.2020.07.013. Epub 2020 Aug 7.

Backbone

• Vector backbone: pET-28b-Cas9-His
• Backbone size w/o insert (bp): 5160
• Total vector size (bp): 8160
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rfx-Cas13d
• Alt name: CasRx
• Species: Ruminococcus flavefaciens XPD3002
• Insert Size (bp): 3000
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pET-28b-hsCas13d.gbk
• A portion of this plasmid was derived from a plasmid made by: Cloned from pT3TS-RfxCas13d
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain) and Bazzini Lab (Stowers Institute, Kansas, MO, USA)