pT3TS-RfxCas13d-NLS-HA
(Plasmid #141321)

• Purpose: Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)
• Depositing Labs: Ariel Bazzini, Miguel Angel Moreno-Mateos
• Publication: Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7. doi: 10.1016/j.devcel.2020.07.013. Epub 2020 Aug 7.

Backbone

• Vector backbone: pT3TS
• Backbone size w/o insert (bp): 3150
• Total vector size (bp): 6150
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rfx-Cas13d (NLS-RfxCas13d-NLS)
• Alt name: CasRx
• Species: Ruminococcus flavefaciens XPD3002
• Insert Size (bp): 3000
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pT3TS-Cas13d-NLS.gbk
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid #109049 used as template as a gift from Patrick Hsu (Konermann et al., 2018).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain) and Bazzini Lab (Stowers Institute, Kansas, MO, USA)