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Addgene

pRATIO4212
(Plasmid #141313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRATIO2212
  • Backbone manufacturer
    Aashima Khosla, David Nelson (unpublished)
  • Backbone size (bp) 14000
  • Modifications to backbone
    nuclear localization sequence (NLS) was removed by digesting with XbaI and SpeI
  • Vector type
    Plant Expression
  • Promoter UBQ10 promoter
  • Selectable markers
    Kanamycin
  • Tags / Fusion Proteins
    • mScarlet-I (C terminal on insert)
    • mVenus (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRATIO4212 was a gift from David Nelson (Addgene plasmid # 141313 ; http://n2t.net/addgene:141313 ; RRID:Addgene_141313)

  • For your References section:

    A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants. Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC. Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. eCollection 2020 Jun. 10.1002/pld3.231 PubMed 32582876
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