pRATIO2212
(Plasmid
#141302)
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Purpose(Empty Backbone) Gateway cloning compatible dual fluorescent ratiometric binary vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGWB401
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Backbone manufacturerTsuyoshi Nakagawa
- Backbone size (bp) 11000
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Vector typePlant Expression
- Promoter UBQ10 promoter
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Selectable markersKanamycin
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Tags
/ Fusion Proteins
- mScarlet-I (C terminal on insert)
- mVenus (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRATIO2212 was a gift from David Nelson (Addgene plasmid # 141302 ; http://n2t.net/addgene:141302 ; RRID:Addgene_141302) -
For your References section:
A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants. Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC. Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. eCollection 2020 Jun. 10.1002/pld3.231 PubMed 32582876
Map uploaded by the depositor.
