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Addgene

pCryptDel4.8
(Plasmid #141293)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141293 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFREE
  • Backbone manufacturer
    Lauritsen et al. Microb. Cell Factories 16, (2017).
  • Total vector size (bp) 7621
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RelB
  • Species
    Escherichia coli Nissle 1917
  • Insert Size (bp)
    142
  • GenBank ID
    WP_001554710
  • Promoter Native promoter from pMUT2 relB/relE operon

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    gRNA targetting pMUT2
  • Species
    Synthetic
  • Insert Size (bp)
    30
  • Promoter Prha (rhamnose inducible promoter)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer cccagtcagctccttccgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCryptDel4.8 was a gift from Neel Joshi (Addgene plasmid # 141293 ; http://n2t.net/addgene:141293 ; RRID:Addgene_141293)

  • For your References section:

    Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917. Kan A, Gelfat I, Emani S, Praveschotinunt P, Joshi NS. ACS Synth Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466. 10.1021/acssynbio.0c00466 PubMed 33301298
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