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PurposeOE of HRI
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141284 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonesafe-harbor-expression
- Backbone size w/o insert (bp) 4359
- Total vector size (bp) 8230
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Vector typeMammalian Expression
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Selectable markersfluorescent
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHRI
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SpeciesH. sapiens (human)
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Insert Size (bp)3871
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Entrez GeneEIF2AK1 (a.k.a. HCR, HRI, LEMSPAD, hHRI)
- Promoter EF1a
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Tag
/ Fusion Protein
- mClover
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site speI (not destroyed)
- 3′ cloning site mluI (not destroyed)
- 5′ sequencing primer cattatacgaagttattcgacattg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXG290 was a gift from Martin Kampmann (Addgene plasmid # 141284 ; http://n2t.net/addgene:141284 ; RRID:Addgene_141284) -
For your References section:
Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway. Guo X, Aviles G, Liu Y, Tian R, Unger BA, Lin YT, Wiita AP, Xu K, Correia MA, Kampmann M. Nature. 2020 Mar;579(7799):427-432. doi: 10.1038/s41586-020-2078-2. Epub 2020 Mar 4. 10.1038/s41586-020-2078-2 PubMed 32132707
Map uploaded by the depositor.
