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PurposeGateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDONR223
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Backbone manufacturerRual et al, Genome Res. 14:2128-2135, 2004.
- Backbone size w/o insert (bp) 5005
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Vector typeGateway-compatible Entry vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNSP7
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Alt nameGene ID 43740578
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SpeciesSARS-CoV-2 isolate Wuhan-Hu-1
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Insert Size (bp)255
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MutationMany synonymous changes due to codon optimization
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthesized from GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223 SARS-CoV-2 NSP7 was a gift from Fritz Roth (Addgene plasmid # 141261 ; http://n2t.net/addgene:141261 ; RRID:Addgene_141261) -
For your References section:
A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP. G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. 10.1534/g3.120.401554 PubMed 32763951