pET20b-delta133HtrA2
(Plasmid #14126)

• Depositing Lab: L. Miguel Martins
• Publication: Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-27.

Backbone

• Vector backbone: pET20b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3584
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: When transformed and plated on LB/agar/Amp plates, this plasmid will normally only produce colonies if the plates are incubated at room temp as opposed to 37oC. Following this, the bacteria when inoculated in liquid media will grow well at 37oC.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HtrA2
• Alt name: Omi
• Species: H. sapiens (human)
• Insert Size (bp): 978
• GenBank ID: NM_013247
• Entrez Gene: HTRA2 (a.k.a. MGCA8, OMI, PARK13, PRSS25)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry