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Addgene

pCAS9c
(Plasmid #141250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCM188/p414-TEF1p-Cas9-CYC1t
  • Backbone manufacturer
    Gari et al., 1997/DiCarlo et al., 2013
  • Modifications to backbone
    Replaced CEN/ARS with 2µ and replaced TRP1 marker with URA3
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAS9c was a gift from Adam Bertl (Addgene plasmid # 141250 ; http://n2t.net/addgene:141250 ; RRID:Addgene_141250)

  • For your References section:

    Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing. Degreif D, Kremenovic M, Geiger T, Bertl A. J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018. 10.14440/jbm.2018.254 PubMed 31453248
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