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Addgene

pPT-PCR C1
(Plasmid #141248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGE3.0
  • Backbone manufacturer
    Brumwell et al. 2019
  • Backbone size w/o insert (bp) 17552
  • Total vector size (bp) 59873
  • Modifications to backbone
    Inserted the P. tricornutum mitochondrial genome into the backbone of pAGE3.0
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology ; Diatom Expression
  • Selectable markers
    HIS3 ; Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300 (Epicentre)
  • Growth instructions
    Optional: Arabinose induction to high plasmid copy number.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Reduced Mitochondrial Genome of Phaeodactylum tricornutum
  • Alt name
    P. tricornutum
  • Alt name
    Mitochondrial Genome
  • Species
    P. tricornutum
  • Insert Size (bp)
    42321
  • Mutation
    Repeat region removed, additional minor mutations described in manuscript
  • GenBank ID
    NC_016739.1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note this plasmid is larger than what is typically sequenced at Addgene. Discrepancies present in the mitochondrial genome sequence are not of any functional concern for the plasmid.

Please visit https://www.biorxiv.org/content/10.1101/2019.12.19.882662v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPT-PCR C1 was a gift from Bogumil Karas (Addgene plasmid # 141248 ; http://n2t.net/addgene:141248 ; RRID:Addgene_141248)

  • For your References section:

    Rapid method for generating designer algal mitochondrial genomes. Cochrane RR, Brumwell SL, Soltysiak MPM, Hamadache S, Davis JG, Wang J, Tholl SQ, Janakirama P, Edgell DR, Karas BJ. Algal Research, 2020. 50, 102014 10.1016/j.algal.2020.102014
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