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Addgene

pDECKO_mCherry_AAVS1
(Plasmid #141212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDECKO_GFP
  • Backbone size w/o insert (bp) 10994
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA
  • gRNA/shRNA sequence
    targets the intron of the AAVS1 gene, 1:actctttaaggaaagaagga 2:cctcagtctgaagagcagagc
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Roderic Guigo & Rory Johnson (Addgene plasmid # 78534)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

derivative of Addgene plasmid # 78534

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDECKO_mCherry_AAVS1 was a gift from Rory Johnson (Addgene plasmid # 141212 ; http://n2t.net/addgene:141212 ; RRID:Addgene_141212)
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