pDECKO_GFP
(Plasmid
#141210)
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Purpose(Empty Backbone) empty GFP vector for cloning sgRNA or pgRNAs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDECKO_GFP
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Backbone manufacturerDerived from Roderic Guigo's & Rory Johnson's pDECKO_mCherry plasmid (Addgene plasmid # 78534)
- Backbone size (bp) 10994
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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A portion of this plasmid was derived from a plasmid made byRoderic Guigo & Rory Johnson (Addgene plasmid # 78534)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
derivative of Addgene plasmid # 78534
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDECKO_GFP was a gift from Rory Johnson (Addgene plasmid # 141210 ; http://n2t.net/addgene:141210 ; RRID:Addgene_141210) -
For your References section:
Multi-hallmark long noncoding RNA maps reveal non-small cell lung cancer vulnerabilities. Esposito R, Polidori T, Meise DF, Pulido-Quetglas C, Chouvardas P, Forster S, Schaerer P, Kobel A, Schlatter J, Kerkhof E, Roemmele M, Rice ES, Zhu L, Lanzos A, Guillen-Ramirez HA, Basile G, Carrozzo I, Vancura A, Ullrich S, Andrades A, Harvey D, Medina PP, Ma PC, Haefliger S, Wang X, Martinez I, Ochsenbein AF, Riether C, Johnson R. Cell Genom. 2022 Aug 22;2(9):100171. doi: 10.1016/j.xgen.2022.100171. eCollection 2022 Sep 14. 10.1016/j.xgen.2022.100171 PubMed 36778670