pMP89b CvTA
(Plasmid
#141204)
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PurposeChromobacterium violaceum transaminase with TEV cleaving site after the N-terminal Histag.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSETb
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCvTA
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SpeciesChromobacterium violaceum
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MutationWT
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMP89b CvTA was a gift from Francesca Paradisi (Addgene plasmid # 141204 ; http://n2t.net/addgene:141204 ; RRID:Addgene_141204) -
For your References section:
Characterization of a novel amine transaminase from Halomonas elongata. Cerioli L, Planchestainer M, Cassidy J, Tessaro D, Paradisi F. J Mol Cat B: Enz 2015 v120 (141-150) 10.1016/j.molcatb.2015.07.009