pRSETB BS2mT4L1
(Plasmid
#141203)
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PurposeChimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141203 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSETb
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBS2m-T4L1
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SpeciesBacillus subtilis
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MutationE11Q
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSETB BS2mT4L1 was a gift from Francesca Paradisi (Addgene plasmid # 141203 ; http://n2t.net/addgene:141203 ; RRID:Addgene_141203) -
For your References section:
Genetically Fused T4L Acts as a Shield in Covalent Enzyme Immobilisation Enhancing the Rescued Activity. Planchestainer M, Padrosa DR, Contente ML, Paradisi F. Catalysts 2018, 8(1), 40 10.3390/catal8010040