pET-DUET-1-mRhubarb720-HO
(Plasmid #141201)

• Purpose: Expresses mRhubarb720 fluorescent protein and heme oxygenase
• Depositing Lab: Elad Firnberg
• Publication: Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10.1038/s41598-019-52123-7.

Backbone

• Vector backbone: pET-DUET-1
• Total vector size (bp): 7064
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mRhubarb720
• Species: Synthetic
• Insert Size (bp): 993
• Mutation: R134H, L196Q, T202D, V203I, W309R, Q310A compared to iRFP
• Promoter: T7
• Tag / Fusion Protein:
  • 6x-His (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: ATG CGT CCG GCG TAG A
• 3′ sequencing primer: GAT TAT GCG GCC GTG TAC AA

Gene/Insert 2

• Gene/Insert name: heme oxygenase
• Insert Size (bp): 831
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: AvrII (not destroyed)
• 5′ sequencing primer: TTG TAC ACG GCC GCA TAA TC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry