pMIG-N19RhoA
(Plasmid #14120)

• Depositing Lab: Richard Hynes
• Publication: Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Backbone

• Vector backbone: pMIG
• Backbone size w/o insert (bp): 6753
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N19 Human RhoA
• Alt name: Dominant Negative RhoA Mutant
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Mutation: Site directed mutagenesis of Thr to Asn at Codon 19
• GenBank ID: NM_001664
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLXSN 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Marc Symons North Shore LIJ Res Inst Oncol and Cell Biol 350 Commnity Drive Manhasset, NY 11030

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Qiu, R.-G., Chen, J., Kirn, D., McCormick, F., & Symons, M. Nature 374, 457-459 (1995), Qiu, R.-G., Chen, J., McCormick, F., & Symons, M. Proc. Natl. Acad. Sci. USA 92, 11781-11785 (1995).