pcDNA4/TO/Strep-HA-ZAKalpha_K45A
(Plasmid #141194)

• Purpose: Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line
• Depositing Lab: Simon Bekker-Jensen
• Publication: Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20)30189-1. doi: 10.1016/j.molcel.2020.03.021.

Backbone

• Vector backbone: pcDNA4/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5078
• Total vector size (bp): 7500
• Modifications to backbone: Strep and HA tags were inserted between HindIII and EcoRV sites
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAP3K20
• Alt name: Mitogen-activated protein kinase kinase kinase 20
• Alt name: MLTK
• Alt name: ZAK
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Mutation: Changed Lysine 45 to Alanine
• Entrez Gene: MAP3K20 (a.k.a. AZK, CNM6, MLK7, MLT, MLTK, MLTKalpha, MLTKbeta, MRK, SFMMP, ZAK, mlklak, pk)
• Promoter: CMV
• Tag / Fusion Protein:
  • Strep, HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BHG-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry