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pcDNA4/TO/Strep-HA-ZAK alpha
(Plasmid #141193)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141193 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 7500
  • Modifications to backbone
    Strep and HA tags were inserted between HindIII and EcoRV sites
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAP3K20
  • Alt name
    Mitogen-activated protein kinase kinase kinase 20
  • Alt name
    MLTK
  • Alt name
    ZAK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    MAP3K20 (a.k.a. AZK, CNM6, MLK7, MLT, MLTK, MLTKalpha, MLTKbeta, MRK, SFMMP, ZAK, mlklak, pk)
  • Promoter CMV
  • Tag / Fusion Protein
    • Strep, HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: Plasmid contains a P777S mutation in ZAK alpha. This mutation does not affect the protein's functionality.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO/Strep-HA-ZAK alpha was a gift from Simon Bekker-Jensen (Addgene plasmid # 141193 ; http://n2t.net/addgene:141193 ; RRID:Addgene_141193)

  • For your References section:

    ZAKalpha Recognizes Stalled Ribosomes through Partially Redundant Sensor Domains. Vind AC, Snieckute G, Blasius M, Tiedje C, Krogh N, Bekker-Jensen DB, Andersen KL, Nordgaard C, Tollenaere MAX, Lund AH, Olsen JV, Nielsen H, Bekker-Jensen S. Mol Cell. 2020 Apr 9. pii: S1097-2765(20)30189-1. doi: 10.1016/j.molcel.2020.03.021. 10.1016/j.molcel.2020.03.021 PubMed 32289254
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