pcDNA4/TO/GFPZNF598-9P-9A
(Plasmid #141192)

• Purpose: Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line
• Depositing Lab: Simon Bekker-Jensen
• Publication: Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 10.1016/j.celrep.2019.03.006.

Backbone

• Vector backbone: pcDNA4/TO/GFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5078
• Total vector size (bp): 8000
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZNF598
• Alt name: E3 ubiquitin-protein ligase ZNF598
• Alt name: Zinc finger protein 598
• Species: H. sapiens (human)
• Insert Size (bp): 2700
• Mutation: all 3 proline repeats are mutated to Alanine (9xP-->9xA)
• Entrez Gene: ZNF598 (a.k.a. HEL2)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BHG-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry