pcDNA4/TO/GFP-ZNF598
(Plasmid
#141191)
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PurposeExpresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4/TO/GFP
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5078
- Total vector size (bp) 8000
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZNF598
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Alt nameE3 ubiquitin-protein ligase ZNF598
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Alt nameZinc finger protein 598
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SpeciesH. sapiens (human)
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Insert Size (bp)2700
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Entrez GeneZNF598 (a.k.a. HEL2)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer BHG-Rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4/TO/GFP-ZNF598 was a gift from Simon Bekker-Jensen (Addgene plasmid # 141191 ; http://n2t.net/addgene:141191 ; RRID:Addgene_141191) -
For your References section:
GIGYF1/2-Driven Cooperation between ZNF598 and TTP in Posttranscriptional Regulation of Inflammatory Signaling. Tollenaere MAX, Tiedje C, Rasmussen S, Nielsen JC, Vind AC, Blasius M, Batth TS, Mailand N, Olsen JV, Gaestel M, Bekker-Jensen S. Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 10.1016/j.celrep.2019.03.006. 10.1016/j.celrep.2019.03.006 PubMed 30917308