pcDNA4/TO/GFP-GIGYF1
(Plasmid #141188)

• Purpose: Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line
• Depositing Lab: Simon Bekker-Jensen
• Publication: Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e4. doi: 10.1016/j.celrep.2019.03.006.

Backbone

• Vector backbone: pcDNA4/TO/GFP
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5078
• Total vector size (bp): 8000
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GIGYF1
• Alt name: GRB10-interacting GYF protein 1
• Alt name: PERQ amino acid-rich with GYF domain-containing protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Entrez Gene: GIGYF1 (a.k.a. GYF1, PERQ1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BHG-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry