pcDNA3-SARS-CoV-2-S-RBD-Fc
(Plasmid #141183)

• Purpose: Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
• Depositing Lab: Erik Procko
• Publication: Chan et al Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5335
• Total vector size (bp): 6685
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1
• Species: SARS coronavirus 2
• Insert Size (bp): 1350
• GenBank ID: YP_009724390.1
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Signal peptide from influenza HA (N terminal on insert)
  • Fc region of IgG1 (a.a. Asp-221 to Lys-447) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-fwd
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.