pAcBac1-3nitroY-A7-RS
(Plasmid
#141173)
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PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcBac
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA7 3nY tRNA synthetase
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MutationP186T L274R N311G C313N
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcBac1-3nitroY-A7-RS was a gift from Ryan Mehl (Addgene plasmid # 141173 ; http://n2t.net/addgene:141173 ; RRID:Addgene_141173)