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Addgene

pBabe Nf2 delta BB
(Plasmid #14117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nf2 delta BB
  • Alt name
    Nf2
  • Species
    M. musculus (mouse)
  • Mutation
    delta BB: Blue Box residues deleted
  • Entrez Gene
    Nf2 (a.k.a. merlin)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe Nf2 delta BB was a gift from Tyler Jacks (Addgene plasmid # 14117 ; http://n2t.net/addgene:14117 ; RRID:Addgene_14117)

  • For your References section:

    Cellular transformation by a FERM domain mutant of the Nf2 tumor suppressor gene. Johnson KC, Kissil JL, Fry JL, Jacks T. Oncogene. 2002 Sep 5. 21(39):5990-7. 10.1038/sj.onc.1205693 PubMed 12203111
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