SNAP-Drp1
(Plasmid #141159)

• Purpose: Mammalian expression and labeling of Drp1
• Depositing Lab: Gia Voeltz
• Publication: Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122.

Backbone

• Vector backbone: pAcGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4527
• Total vector size (bp): 6627
• Modifications to backbone: AcGFP was replaced with SNAP tag using AgeI /XhoI
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DRP1
• Alt name: Drp1, isoform 3
• Alt name: DNM1L
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• GenBank ID: NM_005690
• Entrez Gene: DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
• Promoter: CMV
• Tag / Fusion Protein:
  • SNAP-Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pFastBac Reverse

Resource Information

• Supplemental Documents:
  • SNAP-Drp1-CMV-Forward.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry