GFP-Mfn1-E209A
(Plasmid #141155)

• Purpose: Mammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysis
• Depositing Lab: Gia Voeltz
• Publication: Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122.

Backbone

• Vector backbone: pAcGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4707
• Total vector size (bp): 6933
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MFN 1
• Alt name: mitofusin 1
• Species: H. sapiens (human)
• Mutation: changed Glutamic acid at position 209 to Alanine (E209A)
• GenBank ID: NM_033540
• Entrez Gene: MFN1 (a.k.a. hfzo1, hfzo2)
• Promoter: CMV
• Tag / Fusion Protein:
  • AcGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pFastBac Reverse

Resource Information

• Supplemental Documents:
  • GFP-Mfn1-E209A_Mutagenized.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry